CRISPR Design tools

Publication Data

Publication Curation and Resources for Genome Editing
Genome-editing is a powerful technique that allows creating knock-in/out mutations in any gene and any cell. While curated genome-editing publications here are primarily focused on Crispr-Cas9 technologies, clinical trials, protocols and expression vectors cover a broader technologies including Crispr-Cas9, Zinc finger nuclease (ZFN) and Tale Nuclease (TALEN). In our publication curation, it includes species, genes, gRNA's, in publications

Publication Curation Summary

Clinical trials

Resources

Results per page

SpeciesFilter by Species	Target gene or RegionFilter by Target gene or Region	Editing purposeFilter by Editing purpose	Editing purposeFilter by Editing purpose	Type of toolFilter by Type of tool	Type of toolFilter by Type of tool	gRNA sequencesFilter by gRNA sequences	PubmedFilter by Pubmed
Strongyloides	DAF-12	Knockout via Knockin	Knockout	CRISPR/Cas9	CRISPR - Cas	AGGAGCAGGAAACACAACAGAGG	33602820
Mouse	Nomo1	Knockout via Knockin	Knockout	CRISPR/Cas9 RNP	CRISPR - Cas	CCTGAAGATCGAACCTCCTC;GAGTAACGGGGCGCTTCGCA	31833031
Kiwifruit	AcCEN4 (KX611599.1)	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	CCTAGGGTTGAAGTTCATGGAGG;CCACAGGTTTGTGTTTGTGCTCT	30302894
Human	ALIX	Knockout via Knockin	Knockout	CRISPR/Cas9	CRISPR - Cas	GCAGGCCCAGTACTGCCGCG	29981367
Human	TP53	Knockout	Knockout	CRISPR/Cas9-RNP	CRISPR - Cas	GTGATGGGGACGGGATGCAG;CATAGGGTCCATATCCTCCA	33955355
Water buffalo	EGR1	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	NA	33726807
Rat	Cck	Knockin	Knockin	CRISPR/Cas9	CRISPR - Cas	TAGGGCTACAGCTGCGCCAGAC;AAACGTCTGGCGCAGCTGTAGC,TAGGAGCCGCGGCAGAAGCCTG;AAACCAGGCTTCTGCCGCGGCT	25039742
Mouse	Dmd	Mutation correction	Mutation correction	CRISPR/Cas9	CRISPR - Cas	TCTTTGAAAGAGCAATAAAATGG;ATAATTTCTATTATATTACAGGG;ATTTCAGGTAAGCCGAGGTTTGG	26721683
Mouse	Asxl1	Knockout	Knockout	CRISPR/Cas9-RNP	CRISPR - Cas	GGCTTCCGGAAGCCAGCCAC	29751067
Human	NANOG	Transcriptional activation	Transcriptional regulation	CRISPR/dCas9-VP64	CRISPR - dCas	CGCCAGGAGGGGTGGGTCTA;CCTTGGTGAGACTGGTAGA;GTCTTCAGGTTCTGTTGCT;ATATTCCTGATTTAAAAGT	23892895
Pig	LDLR	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	GGCTGGAAGCATGAAGTCCAG	29207527
Zebrafish	smc3	Knockout	Knockout	TALENs	TALENs	TCTCGAATCTCCGCCAT(LEFT),AGCTGAGCGCAGAGCGA(RIGHT);TATTTTTCAGTCGGCAGAAAT(LEFT),ACTTCTTTTATGGTATGTA(RIGHT)	25503746
Human	NRF2	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	NA	32617133
Human	TMPRSS2	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	NA	33493182
Human	AXIN1	Transcriptional repression	Transcriptional regulation	CRISPRi/dCas9-KRAB	CRISPR - dCas	GCTGGCTCGGGAGGCGATCG;GCTCCGGCCGGCTCTGGCGG;GCGACTCCGGCCGGCTCTGG	31097693
Human	TRAC	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	AGAGTCTCTCAGCTGGTACA	28199983
Human	FBXW7	Knockin	Knockin	CRISPR/Cas9	CRISPR - Cas	NA	33872388
Zebrafish	krt15	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	GGTGCTGGAGGAGGCTTCGG	26048245
Herpes simplex virus type 1	UL42	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	GACGCGTCGGACGCGTCCCT;GTCCCCCGTGGAGGACGCGT;GATTAAGTTCGGCGCCCTGC;GTGCAGGGCGCCGAACTTAA	27362483
Human	NFYA	Knockout	Knockout	CRISPR/Cas9	CRISPR - Cas	GGTCCGTCCTGTCTAAGTCGTCGTC	34782004

Results per page

DiseaseFilter by Disease	PhaseFilter by Phase	Target GeneFilter by Target Gene	Editing ToolsFilter by Editing Tools	InterventionFilter by Intervention	CategoryFilter by Category
Leber Congenital Amaurosis					Others
Hormone Refractory Prostate Cancer	Phase 1	PD-1	CRISPR/Cas9	Biological: PD-1 Knockout T Cells/Drug: Cyclophosphamide/Drug: IL-2	Cancer
Human Immunodeficiency Virus (HIV)	Phase 1	CCR5	ZFN	Drug: ZFN Modified CD4+ T Cells/Drug: Cyclophosphamide	HIV
Stage IV Gastric Carcinoma/Stage IV Nasopharyngeal Carcinoma/T-Cell Lymphoma Stage IV/Stage IV Adult Hodgkin Lymphoma/Stage IV Diffuse Large B-Cell Lymphoma	Phase 1/Phase 2	PD-1	CRISPR/Cas9	Drug: Fludarabine/Drug: Cyclophosphamide/Drug: Interleukin-2	Cancer
Metastatic Non-small Cell Lung Cancer	Phase 1	PD-1	CRISPR/Cas9	Drug: Cyclophosphamide/Other: PD-1 Knockout T Cells/Drug: Interleukin-2	Cancer
Major Depression				Other: Blood sample for genetic purpose	Others
HIV	Phase 1	CCR5	ZFN	Genetic: SB-728mR-HSPC Infusion 3 days following busulfan conditioning	HIV
Esophageal Cancer	Phase 2	PD-1	CRISPR/Cas9	Drug: Cyclophosphamide/Drug: Interleukin-2/Other: PD-1 Knockout T Cells	Cancer
Sickle Cell Anemia/Thalassemia					Others
Human Papillomavirus-Related Malignant Neoplasm	Phase 1	HPV16 E6/E7 and HPV18 E6/E7	TALEN; CRISPR/Cas9	Biological: TALEN/Biological: CRISPR/Cas9	Cancer
B Cell Leukemia/B Cell Lymphoma	Phase 1/Phase 2	CD19(UCART019)	CRISPR/Cas9	Biological: UCART019	Cancer
HIV	Phase 1/Phase 2		ZFN	Genetic: SB-728-T/Genetic: SB-728-T/Genetic: SB-728-T/Genetic: SB-728-T/Genetic: SB-728-T	HIV
Sickle Cell Disease Without Crisis	Phase 1			Drug: Plerixafor	Others
				Other: Exposure: abundance of Immature Myeloid Cells counts in blood samples; Other: Exposure: abundance of Immature Dentritic Cells counts in blood samples; Other: Exposure: abundance of Immature monocytes counts in blood samples; Other: Exposure: abundance of Immature neutrophils counts in blood samples	Others
Mucopolysaccharidosis II	Phase 1	Iduronate 2-Sulfatase (IDS)	ZFN	Biological: SB-913	Others
Cholangiocarcinoma/Hepatocellular Carcinoma/Pancreatic Neoplasm				Genetic: Organoid in vitro culture	Cancer
Metastatic Renal Cell Carcinoma	Phase 1	PD-1	CRISPR/Cas9	Biological: PD-1 Knockout T Cells/Drug: Cyclophosphamide/Drug: IL-2	Cancer
Hemophilia B	Phase 1	Factor 9 gene	ZFN	Biological: SB-FIX	Others
Human Immunodeficiency Virus (HIV)	Phase 1/Phase 2	CCR5	ZFN	Genetic: SB-728mR-T/Genetic: SB-728mR-T/Drug: Cyclophosphamide	HIV
HIV Infection/HIV Infections	Phase 1	CCR5	ZFN	Genetic: SB-728-T	HIV

Resources: Protocol cloning vectors

Results per page

Protocol TitleFilter by Protocol Title	Publication TimeFilter by Publication Time	Filter by null	CategoryFilter by Category	Protocol_fieldsFilter by Protocol_fields
ZFN mRNA Production		MilliporeSigma	ZFN	in vitro transcription
Cel1 Protocols AAVS1		MilliporeSigma	AAVS1	CEL-I assay
Transfection Protocol for Virus Production		MilliporeSigma	ZFN	Transfection
Genome Editing in Plants with CRISPRCas9		MilliporeSigma	CRISPR/Cas9
CRISPR/Cas9 Synergistic Activation Mediator (SAM) pooled human library		MilliporeSigma	CRISPR/Cas9	electroporation
Knockout Zinc Finger Nucleases (ZFN)		MilliporeSigma	ZFN	CEL-I Assay
Cel-1		MilliporeSigma	ZFN	CEL-I assay
A CRISPR/Cas-GFP Vector for Rapid Expression Verification and Enrichment of Genome Edited Cells		MilliporeSigma	CRISPR/Cas9
mRNA Production Protocol		MilliporeSigma	ZFN	in vitro transcription
Tips for Cell Engineering using Cas9-GFP CRISPR plasmids		MilliporeSigma	CRISPR/Cas9
CRISPR/Cas Nuclease RNA-guided Genome Editing		MilliporeSigma	CRISPR/Cas9
Custom Zinc Finger Nuclease (ZFN)		MilliporeSigma	ZFN	CEL-I assay
Creating Transgenic Mice using CRISPR-Cas9 Genome Editing		MilliporeSigma	CRISPR/Cas9
CRISPR/Cas9 Synergistic Activation Mediator (SAM) pooled human library		MilliporeSigma	CRISPR/Cas9	transformation
Generation of Genetically Modified Mice using the CRISPR-Cas9 Genome-Editing System	2017	Publication	CRISPR/Cas9	in vitro transcription
Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis	2017	Publication	TALEN	Transfection
Detecting natural adaptation of the Streptococcus thermophilus CRISPR-Cas systems in research and classroom settings	2017	Publication	CRISPR/Cas9	CRISPR library
GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos	2017	Publication	CRISPR/Cas9	electroporation
Microinjection for transgenesis and genome editing in threespine sticklebacks	2017	Publication	TALEN	microinjection
Microinjection for transgenesis and genome editing in threespine sticklebacks	2017	Publication	CRISPR/Cas9	microinjection

Resources: Protocol cloning vectors

Results per page

NameFilter by Name	SpeciesFilter by Species	Gene EditingFilter by Gene Editing	PromoterFilter by Promoter	Selection MarkerFilter by Selection Marker
pLKO_TRC005	NA	NA	hPGK, hU6(-250)	AMPr
ZFN-KK_Lentivirus_v1-2_Tubulin	NA	NA	PGK, AmpR, T3, U6, T7	AmpR
Lv2-pZDonor	NA	NA	T3, T7	ampR
pCL1162_hPGK-puro_EF1alpha-GFP	NA	NA	hPGK, U6, HuEF1alphapromoter	PuroR, BetaLactamase(AmpR)
cellbio746	Homo sapiens	NA	NA	NA
huLGR5_pZFN2	NA	NA	CMVprmt	KanR
#547pRRLsin.PPT.EF1a_Flag-eGFP.Wpre	NA	NA	EF-1alpha	ampicillinresistance
Lv6-UbC_TUB21-eHiFi-EL	NA	NA	T3, U6, T7, UbC	ampR
pLKO_TRC902	NA	NA	hPGK, InducibleU6	AMPr
Lv3-CMV_AAVS1_15556-EL	NA	NA	T3, T7, CMVprmt	ampR
Lv3-PGK-15590-eKK	NA	NA	T3, T7, PGK	ampR
pLP1-D64V	NA	NA	CMV	AmpR
SHC013_pLKO.1-puro_CMV-TagFP635	NA	NA	hPGK,CMV	PuroR, BetaLactamase(AmpR)
pVax-C2A-3FN_CCR5_8196z	NA	NA	CMVprmt	KanR
SHC011_pLKO.1-puro_CMV-TagYFP	NA	NA	hPGK,CMV	PuroR, BetaLactamase(AmpR)
Lv5k-CMV_eHiFi-EL-L7a	NA	NA	T3, CMV, T7	KanR
AAVS1_hPGK-flag-turboGFP-pA_donor	NA	NA	NA	KanR
Lv4-Ef1a_TUB21-eHiFi-EL	NA	NA	T3, T7, Ef1-a	ampR
pLP1	NA	NA	CMV	AmpR
SHC010_pLKO.1-puro_CMV-TagCFP	NA	NA	hPGK,CMV	PuroR, BetaLactamase(AmpR)

CRISPR Genome-Editing

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