CRISPR Design tools

Crispr Design

CRISPR Design

ORDER CRISPR

CRISPR Input
CRISPR Output

Custom Design
Pre-design Database- KO, Activate, Inhibit

Target Genome *

CRISPR Design Based on *

Gene symbol, Gene ID or Alias

Input sequence(s)

Type of Gene *

Protein-coding Gene

microRNA

long non-coding RNA

Enter Gene symbol, Gene ID or Alias *

* Please select Gene from autosuggestion or else enter Gene Id

Selected Genes:

MegaBLAST Check *

Yes

Input Sequence Format *

FLAT Sequence Format

FASTA Sequence Format

FLAT Sequence Template

FASTA Sequence Template

Target Sequence *

Fasta formatted title will be shortened to 10 characters

LOAD SEQUENCE Clear

5000 characters remaining

Number of Target Sites *

Format *

Cas9 (NGG PAM) gRNA

Cas9D10A Paired Nickase

gRNA Mismatches *

paired Nickase Mismatches *

CRISPR gRNA Libraries *

Genome Knockout: GeCKO Sanger Brunello Pre-designed libraries
Gene Regulation: CRISPRa CRISPRi

Submit

Megablast results

Selected gRNA
Potential OFF-Target Sites

Target ID	Target Sequence	Target Sequence:5'-3' (does not include PAM)	PAM	Sense(+) AntiSense(-)	Efficiency	Specificity	0	1	2	3
No Records Found

Design ID	Target Sequence	Off-Target Sites	Mismatch Count	More
No Records Found

Selected gRNA
Primer(s)

Target ID	Target Sequence:5'-3' (does not include PAM)	PAM	Sense(+) AntiSense(-)	Gene Symbol	Gene ID	Efficiency	Specificity	No. of sites in genome 1, 2 or 3 nt different from target sequence
Target ID	Target Sequence:5'-3' (does not include PAM)	PAM	Sense(+) AntiSense(-)	Gene Symbol	Gene ID	Efficiency	Specificity	0	1	2	3
No Records Found

Target ID	LEFT SEQUENCE	RIGHT SEQUENCE	LEFT TM	RIGHT TM	More
No Records Found

Selected Paired Nickase
Primer(s)

Left-arm Design ID	Left-arm Sequence	Right-arm Design ID	Right-arm Sequence	Distance(nt)
No Records Found

Target ID	LEFT SEQUENCE	RIGHT SEQUENCE	LEFT TM	RIGHT TM	More
No Records Found

Left-arm Design ID	Left-arm Sequence	Right-arm Design ID	Right-arm Sequence	Distance(nt)
No Records Found

DesignID_A	offtarget_A	DesignID_B	offtarget_B	Distance	More
No Records Found

Design ID	Target Sequence	Potential off-target Sites	Mismatch Count	More
No Records Found